Coding

Part:BBa_K4728005

Designed by: Michelle Li   Group: iGEM23_Queens-Canada   (2023-10-08)


TphB (Dehydrogenase)

TphB encodes for the DCD dehydrogenase- a critical element of TPA degradation. The dehydrogenase component funtions with the oxygenase (TphA2 and TphA3) and reductase (TphA1) components of TPADO to facilitate this degradation(Sasoh et al., 2006).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 445
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Sasoh, M., Masai, E., Ishibashi, S., Hara, H., Kamimura, N., Miyauchi, K., & Fukuda, M. (2006). Characterization of the terephthalate degradation genes of Comamonas sp. strain E6. Applied and environmental microbiology, 72(3), 1825–1832. https://doi.org/10.1128/AEM.72.3.1825-1832.2006

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